| 质粒类型: | 慢病毒载体 | 
|---|---|
| 高拷贝/低拷贝: | 高拷贝 | 
| 克隆方法: | 多克隆位点,限制性内切酶 | 
| 载体大小: | 7032 bp (查看载体序列) | 
| 5' 测序引物及序列: | GACTATCATATGCTTACCGT | 
| 载体抗性: | Ampicillin (氨苄青霉素) | 
| 筛选标记: | Puromycin | 
pLKO.1-puro is a transfer and cloning vector of shRNA, originally derived from HIV-1. It contains all the necessary cis-elements for packaging, reverse transcription, and integration, which are required for subsequent production of the lentiviral particles.
AgeI and EcoRI (only these two sites available)