| 质粒类型: | 酿酒酵母表达载体 | 
|---|---|
| 表达水平: | 高拷贝 | 
| 诱导方法: | 半乳糖 | 
| 启动子: | GAL1 | 
| 克隆方法: | 多克隆位点,限制性内切酶 | 
| 载体大小: | 5963 bp (查看载体序列) | 
| 5' 测序引物及序列: | GAL1-F: AATATACCTCTATACTTTAACGTC | 
| 3' 测序引物及序列: | CYC1-R: GCGTGAATGTAAGCGTGAC | 
| 载体标签: | C-His, C-V5 | 
| 载体抗性: | Ampicillin (氨苄青霉素) | 
| 筛选标记: | URA3 | 
| 备注: | 利用半乳糖诱导蛋白在酿酒酵母中表达。 | 
pYES2/NT and pYES2/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Both vectors offer the URA3 gene for selection in yeast. pYES2/NT features an N-terminal Xpress™ epitope for detection with Invitrogen's Anti-Xpress™ Antibody and a polyhistidine (6xHis) tag for purification with nickel-chelating resin. pYES2/CT contains a C-terminal V5 epitope for detection and a polyhistidine (6xHis) tag for purification.