pYES3/CT 载体

基本信息

质粒类型: 酿酒酵母表达载体
表达水平: 高拷贝
诱导方法: 半乳糖
启动子: GAL1
克隆方法: 多克隆位点，限制性内切酶
载体大小: 5870 bp (查看载体序列)
5' 测序引物及序列: GAL1-F: AATATACCTCTATACTTTAACGTC
3' 测序引物及序列: CYC1-R: GCGTGAATGTAAGCGTGAC
载体标签: C-His, C-V5
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: TRP1
备注: 利用半乳糖诱导蛋白在酿酒酵母中表达

订购信息

产品编号 产品名称 规格 价格
VT1766 pYES3/CT 2ug 点击询价

质粒图谱

pYES3/CT 质粒图谱

载体描述

pYES3/CT and pYES6/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Vectors feature a C-terminal V5 epitope for detection with an Anti-V5 Antibody. pYES3/CT carries the TRP1 selection marker for selection in yeast. pYES6/CT carries the bsd resistance gene for selection with the antibiotic Blasticidin, a potent selection agent that can be used at very low concentrations and in any strain regardless of auxotrophic marker.

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